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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Sex Med. 2014 Dec 11;12(2):313–327. doi: 10.1111/jsm.12760

Figure 1. The content of activated myofibroblasts in the non-stimulated PD culture (PD−) was not higher than in the non-stimulated non-PD tunica albuginea culture (TA−), as shown by the expression of ACTA2 protein.

Figure 1

PD− and TA− cell cultures maintained in regular DMEM/20% fetal calf serum for 8-15 passages in the absence of added TGFβ1 were essentially fibroblast cultures, as assayed with vimentin, whereas CC− cultures were essentially SMC, as assayed with calponin (24-26). Cells on monolayer in 6-well plates were homogenized from triplicate wells for each culture and subjected to quantitative western blot assay for ACTA2 expression, correcting by GAPDH expression. In the case of the CC− cultures, ACTA2 does not discriminate myofibroblasts from SMC. *p<0.05