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. 2014 Dec 20;34(3):307–325. doi: 10.15252/embj.201489847

Figure 3.

Figure 3

Crystal structure of Ser65-phosphoUb
  1. Structure of phosphoUb in space group P1 with 8 molecules in the asymmetric unit (Table1). Ub molecules are colored differently, and atoms for pSer65 are shown as spheres with red oxygen and purple phosphorous atoms.
  2. Six of eight phosphoUb molecules form similar dimer interactions, where phosphoUb molecules interact via their Ile44 patch. This resembles the crystal structure of Lys48-linked diUb [pdb-id 1aar (Cook et al, 1992)]. Atoms for pSer65 are shown as in (A), and the Ile44 side chain is shown as blue sticks.
  3. Representative 2|Fo|-|Fc| electron density, contoured at 1 σ, covering the phosphorylation site. pSer65 is shown in ball-and-stick representation, and neighboring side chains are shown in stick representation with red oxygen and blue nitrogen atoms.
  4. In wt Ub (left), Ser65 forms a hydrogen bond with the backbone amide of Gln62. Upon phosphorylation (right), an oxygen atom from the phosphate forms the same backbone hydrogen bond.
  5. Cartoon representation and electrostatic surface potential of Ub (left) and phosphoUb (right) in identical orientations. Electrostatics were calculated using CHARMM (www.charmm-gui.org) and are colored from red (negative potential) to blue (positive potential).