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. 2014 Dec 23;34(3):326–343. doi: 10.15252/embj.201489184

Figure 1.

Figure 1

USP45 co-immunoprecipitates with components of the SLX4 complex
  1. Schematic representation of the domain architecture of human USP45 protein.
  2. Endogenous USP45 was immunoprecipitated (IP) from wild-type (WT) and USP45 knockout (KO) KBM7 cells lines. The immunoprecipitates were resolved on a polyacrylamide gel and stained with Instant Blue. The gel was divided into the indicated sections, and proteins identified by mass spectrometry are detailed in Supplementary Table S1. The key proteins that we focus on in this study are indicated.
  3. Endogenous USP45 was immunoprecipitated from the WT and KO KBM7 cell lines and subjected to immunoblotting with the indicated antibodies.
  4. Endogenous USP45 was immunoprecipitated from WT and USP45 KO U2OS cells lines and analysed as in (B). Mascot scores of the interactors are detailed in Supplementary Table S2.
  5. Endogenous USP45 was immunoprecipitated from the WT or KO U2OS cell lines and subjected to immunoblotting with the indicated antibodies. Similar results were obtained in at least two separate experiments.
  6. Schematic representation of the SLX4 complex.

Source data are available online for this figure