Figure 9.

USP45 recruitment to sites of DNA damage is rapid and transient

Live-cell fluorescence analysis of U2OS cells stably expressing either GFP^NLS empty, wild-type (WT) USP45 or catalytically inactive USP45 [Cys199Ala]. Cells were treated with BrdU (10 μM, 24 h), psoralen or angelicin (25 μM, 3 h) and images captured at the indicated times after laser micro-irradiation. The location of the micro-irradiation stripe in each cell is indicated with a yellow line. Similar results were obtained in at least two independent experiments. Scale bar, 10 μm.