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. 2015 Jan 12;34(3):410–424. doi: 10.15252/embj.201488947

Figure 5.

Figure 5

CtIP is not required for degradation of the single-stranded telomeric overhang generated by TRF2 inactivation
  1. Immortalized MEFs were infected with retroviruses expressing the indicated shRNAs, followed by selection with puromycin for 72 h. Cell extracts were prepared 48 h later and analysed by Western blotting as indicated. SMC1 and GAPDH were used as loading controls. *non-specific band.
  2. MboI- and AluI-digested DNA from cells treated as in (A) was resolved by pulsed-field gel electrophoresis and probed with end-labelled (AACCCT)4 probe. Representative pulsed-field gel samples run under native and denatured conditions are shown.
  3. Quantification of the 3′ overhang in cells treated as in (B). For each sample, the ss/total DNA ratios were expressed relative to the GFP shRNA-treated control. Error bars represent SD of two independent experiments.