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. 2015 Feb 24;10(2):e0117392. doi: 10.1371/journal.pone.0117392

Fig 3. Pulse-field gel electrophoresis of Bradyrhizobium sp. DOA9 genomic DNA.

Fig 3

DOA9 cells were digested in 1% pulse field grade (PFG) agarose plugs with proteinase K, as described in the Materials and Methods, and run 0.8% certified megabase agarose in TAE buffer to separate fragments of 225–6,000 kb (A), or 1% certified megabase agarose in 0.5 x TBE buffer (B) to separate fragments of 225–2,200 kb, respectively. Closed arrowheads and open arrowheads indicate the putative chromosome and the plasmid, respectively. Lane M1: PFGE marker, 3.5–5.7 Mb, Saccharomyces pombe chromosomal DNA. Lane M2: low-range (2.03–194 kb) PFG marker DNA ladder. DOA9: DOA9 genomic DNA.