Fig 7. Course of infection in immunized mice with S. aureus isolate P skin infection.

Mice (n = 4) were immunized with the octa-valent vaccine containing IsaA-His₆, LytM-His₆, Nuc-His₆, His₆-pro-Atl, and PSMα1-4, or with placebo. Animals were infected by intradermal inoculation of S. aureus isolate P (3 × 10⁷ CFU), and were monitored for 7 days. (A) Animal body weight over time after S. aureus skin infection was not affected by vaccination (P > 0.05; Quade’s rank analysis of covariance). (B) Lesion size was significantly reduced by vaccination, as indicated by the asterisk (P = 0.005; Quade’s rank analysis of covariance). (C) S. aureus load in the skin lesion at day 7 was not reduced by vaccination (P > 0.05; Mann-Whitney U test).