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ΔTm values of PAs with cognate site TGGAGA and five mutant sequences*.
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The error of these ΔTm values is ± 0.2 °C based on experimental reproducibility. The color for ΔTm is given based on the comparison with the value for PA-cognate DNA complex: < 50% (red), 50% ~ 80% (orange), 80% ~ 100% (green), and > 100% (blue) of ΔTm for PA-cognate DNA complex.
