. Author manuscript; available in PMC: 2015 Oct 14.

Published in final edited form as: Org Biomol Chem. 2014 Oct 14;12(38):7523–7536. doi: 10.1039/c4ob01456a

Table 2.

SPR analysis of kinetic rate constants and equilibrium affinities for PAs binding to their cognate site TGGAGA at 25 °C with 50 mM NaCl^*. ΔTm values with cognate DNA are included for comparison.

PA	Kinetics		K_D (nM)
PA	k_a (×10⁶M⁻¹s⁻¹)	k_d (×10⁻³s⁻¹)	Kinetic fit	Steady state	ΔT_m (°C)
KA1033	4.7 ± 0.7	12 ± 1	2.5 ± 0.4	2.8 ± 0.3	7.8
KA1011	2.6 ± 0.3	96 ± 6	36.4 ± 0.4	36.6 ± 0.1	2.0
KA1013	3.7 ± 0.4	45 ± 7	12.1 ± 0.4	10.3 ± 0.5	5.4
KA2127	1.8 ± 0.3	4.7 ± 0.3	2.6 ± 0.2	2.9 ± 0.2	8.0
KA2128	0.7 ± 0.2	13 ± 2	18.6 ± 0.8	17.5 ± 0.1	3.6
KA2129	1.1 ± 0.2	29 ± 5	26.3 ± 2.2	24.0 ± 0.5	3.2
KJK6021	1.3 ± 0.2	2.6 ± 0.2	2.0 ± 0.2	2.2 ± 0.3	8.8

Errors listed are the standard errors for the fits.