. Author manuscript; available in PMC: 2015 Oct 14.

Published in final edited form as: Org Biomol Chem. 2014 Oct 14;12(38):7523–7536. doi: 10.1039/c4ob01456a

Table 4.

SPR analysis of kinetic rate constants and equilibrium affinities as a function of temperature for KA1033 and KJK6021 binding to TGGAGA with 200 mM NaCl^*.

	KA1033
Temp. (°C)	k_a(×10⁶ M⁻¹s⁻¹)	k_d (×10⁻³s⁻¹)	K_D (nM)	ΔG(kcal mol⁻¹)
18	1.4 ± 0.2	6.9 ± 0.3	5.0 ± 0.4	−11.1
25	1.8 ± 0.4	15 ± 2	8.7 ± 0.5	−11.0
32	2.2 ± 0.3	30 ± 4	14 ± 0.5	−11.0
40	3.0 ± 0.6	52 ± 6	18 ± 0.8	−10.8
	KJK6021
Temp. (°C)	k_a(×10⁵ M⁻¹s⁻¹)	k_d (×10⁻³s⁻¹)	K_D (nM)	ΔG(kcal mol⁻¹)
18	2.9 ± 0.3	2.0 ± 0.2	6.8 ± 0.5	−10.9
25	5.0 ± 0.4	4.5 ± 1.0	9.0 ± 0.4	−11.0
32	8.7 ± 0.6	11 ± 2	13 ± 0.8	−11.0
40	18 ± 1	25 ± 4	14 ± 0.06	−11.0

Errors listed are the standard errors for the 1:1 global kinetic fits.