Fig. 1.
(a) DNA sequences used to simultaneously test binding of multiple sequences. ATATAT, AAATTT, ATGA, and R1. Concentration of DNA is 5μM. (b-d) Spectra of DNA and Net binding with increasing ligand concentration. Ligand to DNA ratios are [0:1], [1:1], and [2:1], respectively. (e-g) Spectra show the competitive binding of Net and DB75 with multiple DNA sequences. Respective ratios shown at [0.5:1], [1:1], and [2:1]. Free DNA is indicated by sequence name above corresponding peak (AAATTT, m/z 7,921.5) and ligand-DNA complex as “name + n” (AAATTT + Net, m/z 8,352.5)