Table 1.

The dsDMR enhancers validated in vivo

dsDMR enhancer	Adjacent gene	Location from TSS (kb)	Related enriched GO terms	Expression pattern of adjacent gene at 24hpf
six3a-e1	six3a	−18.6	brain development, central nervous system development	forebrain, midbrain hindbrain boundary, anterior neural tube
six3a-e2		−50.4
six3b-e1	six3b	−104.2	brain development, central nervous system development, eye development, sensory organ development	immature eye, diencephalon, telencephalon, forebrain, optic vesicle
six3b-e2		−35.6
fgfr2-e1	fgfr2	−3.7	positive regulation of cell proliferation, fibroblast growth factor receptor signaling pathway	forebrain, hindbrain, diencephalon, central nervous system, solid lens vesicle
fgfr2-e2		−8.6
fgfr2-e3		−38.8
fgfr2-e4		−107.5
fgf3-e1	fgf3	−54.2	brain development, sensory organ development, dorsal/ventral pattern formation, cell fate specification, regulation of neurogenesis	forebrain, midbrain, midbrain hindbrain boundary, somite, diencephalon, optic stalk
znf703-e1	znf703	−37.1	brain development, central nervous system development, eye development, sensory organ development	hindbrain, midbrain, neural tube, somite, spinal cord, optic cup, vein
znf703-e2		−74.7
znf703-e3		−78.8
sox2-e1	sox2	219.2	eye development, sensory organ development, dorsal/ventral pattern formation, regulation of transcription factor activity	immature eye, midbrain, neural tube, spinal cord, forebrain, hindbrain, telencephalon, cerebellum, octic vesicle
sox2-e2		68.0
sox2-e3		64.5
sox2-e4		20.3
sox2-e5		−47.1
wnt3-e1	wnt3	−3.1	Wnt receptor signaling pathway	midbrain, hindbrain, cerebellum, diencephaoln
wnt3a-e1	wnt3a	44.5	brain development, neural tube development, dorsal/ventral pattern formation, Wnt receptor signaling pathway	neural crest cell, midbrain, cerebellum, diencephalon
wnt3a-e2		37.0