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. 2015 Feb 11;8:9. doi: 10.1186/s13041-015-0101-6

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© Choi et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overview of double immunostaining design. (A) Diagram of the initial experiments comparing early proliferation of astrocytes (BrdU/GFAP-positive) at 1 week and mature neuron formation at 4 weeks (BrdU/NeuN-positive) after middle cerebral artery occlusion (MCAO). (B) Coronal sections delineating the main areas of interest: frontal and temporal boundary zone.