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. 2015 Feb 11;8:9. doi: 10.1186/s13041-015-0101-6

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© Choi et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunohistochemical (IHC) staining for anti-nestin in the peri-infarct area at 3, 7, and 14 days after ischemia (magnification, A 40×; B 400×). Compared with the corresponding regions of the control group, there was an increase in nestin-positive cells in the aLA group at 3, 7, and 14 days after the MCAO. Nestin-positive cells in the aLA group exhibited a variety of morphological features such as stellar shape, large cell body, and multiple processes (arrow). In contrast, stained cells in the control group exhibited small cell bodies, vacuolization, degradation, and loss of processes (arrowhead). n = 3 rats/day/group.