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. Author manuscript; available in PMC: 2015 Feb 25.
Published in final edited form as: Stem Cells. 2014 Dec;32(12):3209–3218. doi: 10.1002/stem.1809

Figure 1. EMT-TF, SNAI2 is highly expressed in epidermal progenitor cells and downregulated during differentiation.

Figure 1

(A) RNA-seq analysis on the expression of EMT-TF family members in epidermal keratinocytes. Expression levels are represented as reads per kilobase per million (RPKM). Data was derived from the ENCODE RNA seq analysis of human epidermal keratinocytes. (B) RT-qPCR for expression of EMT genes. Due to the high levels of SNAI2 expression, the expression of all other EMT genes were calculated as a percentage of SNAI2 expression. (C) RT-qPCR for expression of SNAI2 in progenitor cells (cultured in growth medium: GM) and differentiated cells (cultured in differentiation medium: DM). Expression levels were normalized to GADPH. Error bars=mean with SEM. (D) Western blot for protein levels of SNAI2 in progenitor (GM) and differentiated cells (DM). Actin was used as a loading control. (E) Quantitation of Western blots shown in (D). Signals were normalized to actin loading control and quantitated using ImageJ. N=3 independent experiments. (F) SNAI2 staining in adult human epidermis. SNAI2 staining is shown in green and differentiation protein keratin 10 (K10) is shown in red. Hoechst staining in blue marks the nuclei. Scale bar=25μm; dashed lines denote basement membrane zone.