Validation of injection parameters. A, CtB+ cell bodies in the AcbSh and LS regions after (B) CtB injection into the LH. C, Acute AAV infusions are restricted to the AcbSh region, without diffusion into the adjacent LS. D, Optic cannula implanted just dorsal to AcbSh terminal regions in the LH. E, F, Histology panels showing regions of AAV viral infusions in the AcbSh (left) and optic cannula placement (right) for global AcbSh (E) and selective AcbSh-LH (F) stimulation groups. Images shown at 4× magnification.