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. 2015 Feb 25;35(8):3537–3543. doi: 10.1523/JNEUROSCI.1524-14.2015

Figure 4.

Figure 4.

In vitro validation of stimulation parameters. The stimulation paradigm used for in vivo experiments was tested in slices of the AcbSh (A, B) and LH (C, D). A, EYFP+ neuron in an AcbSh slice recorded in current-clamp; current step delivery confirms neuron health and medium spiny neuron identity. B, Optogenetic stimulation of the neuron in A produces reliable action potential firing with the in vivo 10 s 20 Hz burst paradigm, repeated every 30 s for 10 min to confirm response stability. C, LH neuron recorded in a slice containing dense EYFP terminal labeling responds to current step delivery. D, Voltage-clamp recording of the neuron in C showing inward IPSCs in response to optogenetic AcbSh-LH terminal stimulation for 10 s at 20 Hz in the presence of kynurenic acid.