. 2015 Feb 25;35(8):3625–3643. doi: 10.1523/JNEUROSCI.3388-14.2015

Table 2.

Effects of cholinergic drugs on afferent responses to efferent stimulation

Drug^a	Range of drug concentrations used (μm)	CD				BT/BM
Drug^a	Range of drug concentrations used (μm)	n	Control (spikes/s)^b	Drug (spikes/s)^b	%Block^c	n	Control (spikes/s)^b	Drug (spikes/s)^b	%Block^c
α9α10 nAChR antagonists
αBTX	1–3	4	50.8 ± 9.6^*	45.2 ± 10.6^*	11	6	−26.1 ± 3.7^**	0.6 ± 1.6	102^**
α-CtxRgIA	0.3–1.67	3	48.6 ± 4.6^**	56.8 ± 5.3^**	−17	12	−26.9 ± 2.1^**	−2.1 ± 3.1	92^**
ICS	10	11	39.3 ± 4.8^**	27.3 ± 4.0^**	31^**	16	−30.1 ± 2.4^**	−1.0 ± 1.5	97^**
MLA	0.1–3	3	47.2 ± 6.8^*	5.0 ± 1.6	89^*	11	−26.6 ± 1.3^**	−1.1 ± 0.8	96^**
Afferent nAChR antagonists
α-CtxMII	1–2	6	43.1 ± 8.9^**	9.4 ± 2.7^*	78^**	6	−26.3 ± 4.7^**	−24.7 ± 4.7^**	6
bPiDDB	0.1–3	11	44.0 ± 6.5^**	−4.2 ± 2.2	110^**	5	−30.4 ± 4.6^**	−30.8 ± 4.8^**	−1
DMPP	5–20	27	51.1 ± 4.2^**	−5.6 ± 2.3^*	109^**	10	−22.1 ± 5.4^**	−25.4 ± 3.4^**	−15
5-iodo	0.01–3	4	62.8 ± 19.7^*	4.8 ± 8.1	92^*	5	−36.1 ± 7.0^**	−35.5 ± 7.7^**	2
SazA	0.01–3	5	41.4 ± 4.2^**	−2.6 ± 2.8	106^**	5	−36.1 ± 4.3^**	−35.2 ± 3.2^**	2
Varenicline	0.1–1	4	33.1 ± 8.6^*	3.9 ± 3.4	88^*	5	−28.9 ± 4.2^**	−29.5 ± 3.6^**	−2

^aDrug effects on average spike responses in CD and BT/BM afferents, taken from the first 100 ms interval immediately following the efferent shock train (20 shocks at 200/s). Drugs were classified as either α9α10 or afferent nAChR antagonists.

^bMean discharge rate during shock train minus unstimulated background rate, before and after the indicated drug, respectively. Reported values are mean ± SEM in the absence (Control) and presence (Drug) of identified cholinergic agent. A one-sample t test was used to evaluate whether these means differed from zero; level of significance indicated by asterisk.

^c100 * (1 − Drug/Control) where: 0–100%, drug response is smaller but of same sign as control response; >100%, drug response of opposite sign from control response; <0%, drug response larger but of same sign as control; statistical tests for %Block, paired t test between Drug and Control. For all statistical tests, asterisks indicate level of significance:

*p < 0.05;

**p < 0.01.