. 2015 Feb 25;35(8):3625–3643. doi: 10.1523/JNEUROSCI.3388-14.2015

Table 3.

Effects of afferent nAChR blockers on ensemble responses to efferent stimulation

Unit	Drug (concentration, μm)^b	n	Measure^a
			Voltage (mV)^c			Variance^d
			Control^e	Drug^e	%Block^f	Control^e	Drug^e	%Block^f
BT/BM	bPiDDb (0.3), DMPP (10–20), 5iodo (0.3), SazA (0.1)	9	−0.25 ± 0.32	−1.64 ± 0.34^**	313^**	−0.68 ± 0.11^**	−0.85 ± 0.05^**	24
CD_C	α-CtxmII (2), bPiDDb (0.3), DHβE (0.3–1), DMPP (20), SazA (0.01–0.3)	14	0.83 ± 0.08^**	0.13 ± 0.02^**	84^**	−0.01 ± 0.03	−0.02 ± 0.02	−81
CD_D	DHβE (0.1–1), DMPP (20), 5iodo (0.03), SazA (0.1)	9	1.16 ± 0.28^**	−0.14 ± 0.21	112^*	−0.27 ± 0.08^**	−0.37 ± 0.09^**	−33
CD_E	bPiDDb (0.3), DHβE (0.3–1), CYT (0.3), DMPP (5–20), 5iodo (0.3)	10	0.95 ± 0.12^**	0.17 ± 0.03^**	83^**	0.41 ± 0.10^**	−0.02 ± 0.02	104^**

^aDrug effects on ensemble mean and variance responses in CD and BT/BM afferents, from the first 80 ms interval taken 20 ms after the efferent shock train (20 shocks at 200/s).

^bSpecific drugs and concentrations used in each unit subclass.

^cAverage voltage during shock train minus background voltage.

^d(Var_eff/Var_back) − 1, where Var_back and Var_eff are average ensemble variances before and during the train, respectively.

^eReported values for control and drug columns are means ± SEM in the absence (Control) and presence (Drug) of drugs that selectively block afferent nAChRs. Mean values for CD entries were pooled from units where afferent nAChR antagonists blocked >70% of the efferent-mediated depolarization. Mean values for BT/BM units were pooled from recordings under comparable pharmacological conditions, including concentrations and time of exposure. For each unit, ensemble measurements were tabulated from 10–25 efferent shock presentations under both control and drug conditions. A one-sample t test was used to evaluate if these means differed from zero; level of significance indicated by asterisk.

^f100 * (1 − Drug/Control) where: 0–100%, drug response smaller but of same sign as control response; >100%, drug response of opposite sign from control response; <0%, drug response larger but of same sign as control; statistical tests for %Block, paired t test between Drug and Control. For all statistical tests, asterisks indicate level of significance:

*p< 0.05;

**p< 0.01.