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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Nat Neurosci. 2015 Feb 2;18(3):402–407. doi: 10.1038/nn.3936

Figure 2. Acute whisker stimulation leads to an increase in spine sGluA1 in vivo in apical dendrites of layer 2/3 neurons in the barrel cortex.

Figure 2

a, Long-term stable expression of SEP-GluA1 and dsRed2. Same spines were marked with arrows and arrowheads in different imaging sessions. b, Representative time-lapse in vivo 2-photon images of layer 2/3 pyramidal cell apical tuft dendrites taken with no whisker stimulation (Control) or before (Stimulated, hour 0) and after 1-hour-long acute whisker stimulation (Stimulated, hour 1, 2, and 3). Arrowheads mark spines and arrows mark dendritic shafts (SEP-GluA1 in green, dsRed2 in magenta, overlap in white). Images are single plane median filtered images that were up-scaled and contrast enhanced.