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. 2015 Feb 24;10(2):e0116560. doi: 10.1371/journal.pone.0116560

Fig 4. Impact of the down-secretion of CC chemokines by DEPe on the chemotaxis of CCR4+ cells.

Fig 4

(A) Graph from flow cytometry showing the CCR4 membrane expression of H9 cells. (B) The number of H9 cells which migrated in the presence of conditioned media of unpolarized MΦ (Unpol.MΦ) or of MΦ exposed to 10 μg/ml DEPe or to DMSO (UNT) during M2 polarization was evaluated by transwell migration assay. Data, representing 3 independent experiments, are expressed in number of cells migrated and are evaluated by cell count. **p<0.01.