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. 2015 Feb 24;10(2):e0116560. doi: 10.1371/journal.pone.0116560

Fig 5. Effects of LPS on cytokine secretion in DEPe-exposed MΦ.

Fig 5

Six-day cultured M-CSF MΦ were activated with IFNγ or with IL-4 to obtain M1 and M2 MΦ, respectively, in the presence of 10 μg/ml DEPe or DMSO during 24 h. LPS (10 ng/ml) was next added to DEPe-M1 or DEPe-M2 MΦ for additional 24 h. Cytokine levels in culture medium were determined by ELISA. Data expressed in ng/ml (A) or (B) in ratio of IL-12p40 and IL-10 secretion levels (a.u: arbitrary unit) are the means ± SEM of 6 independent experiments. *p<0.05 when compared with its LPS counterpart, $p<0.05 and $$p<0.01 when compared to M1 LPS MΦ. ns: not significant.