Histograms are shown that illustrate the effect of diet and E treatment on gene expression of CRF receptors and UCN1 in Ovx marmosets. CRF-R1 exhibited a significant effect of E treatment (p=0.040), but no effect of diet and no interaction between E treatment and diet. Cohen’s D analysis of placebo versus E treatment in each diet group showed a large effect size equal to 0.626 and 0.557, for LFD and HFD, respectively. CRF-R2 expression showed significant effects of E treatment and diet with a significant interaction between the two (p=0.017, 0.014 and 0.007, respectively). There was a significant increase in CRF-R2 in the E treated group compared to the control group in the LFD cohort (Bonferroni p<0.01), but not in the HFD cohort. UCN1 expression was significantly suppressed by the HFD diet (t test p=0.045), but it was not affected by E treatment.
*statistically different, Bonferroni’s posthoc test or t-test
# 2-way ANOVA (F[1,11]=4.52; p=0.057)