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. 2014 Dec 20;15:586. doi: 10.1186/s12865-014-0062-z

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© Luo et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Efficient release of nuclei from cells using hypo-osmotic buffer and pipetting. Phase-contrast microscopic image of THP-1 cells in Lysis Buffer before (A) and after (B) pipetting through a 200-μl pipette tip. Original magnification: ×200. Arrowheads indicate membrane components around the nuclei that were observed before (A), but not after (B), pipetting.