Figure 4. Cell volume does not change through the reversible clearing process.

(A) Cell morphology of a representative cell stained with DiI remained qualitatively unchanged following clearing and unclearing. (B) The volume of individual chondrocytes was computed across the three conditions. There was no significant change in average cell volume (n=10) in control (399.6±218.9 mm³), cleared (390.6±218.4 mm³), and uncleared samples (402.4±216.8 mm³) (p>0.050). Cleared samples typically provided better definition of membrane structure, with less scatter, as indicated by the improved definition of membrane morphology and the slight reduction in normalized volume.