Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Osteoarthritis Cartilage. 2014 Nov 29;23(3):405–413. doi: 10.1016/j.joca.2014.11.021

Figure 5. Fluorescent labeling was observed in cartilage from the medial condyle by confocal microscopy at a depth of 200 μm.

Figure 5

(a) The characteristic morphology of chondrocytes at the superficial articular surface and deep zones near the osteochondral interface was revealed by staining for nuclei (Hoechst, blue) and actin filaments (phalloidin, green). Immunolabeling of superficial zone protein (aka SZP/lubricin/PRG4; white arrows) and fibrillin-2 was observed at the articular surface, although it is not clear whether penetration of the antibodies through the dense matrix was achieved. (b) Immunolabeling of perlecan (green), showing increased volume of the pericellular matrix compared to the actin-labeled cell volume alone (yellow and red arrows). Immunolabeling for types II and VI collagen could not be observed at the imaging depth of 200 μm. Scale bars = 20 μm.