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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Free Radic Biol Med. 2014 Nov 28;79:56–68. doi: 10.1016/j.freeradbiomed.2014.11.016

Fig. 5. Protein S-glutathionylation of the 51 kDa and 75 kDa subunits of Complex I in myocardial mitochondria and the effect of eNOS knockout.

Fig. 5

A mitochondrial preparation (1 mg protein used per IP) was subjected to immunoprecipitation (IP) with Ab51 and Ab75 (Ab against 51 kDa and 75 kDa in [6, 41] respectively), and subsequently subjected to SDS-PAGE and immunoblotted with anti-GSH monoclonal antibody (1:500, upper panel). Blottings on the nitrocellulose membrane were probed with Ab 51 (1:10,000) and Ab75 (1:5000) to quantitate the protein of the 51 kDa and 75 kDa subunits. The density ratios of signals were quantitated by software Image J (NIH) (n = 3, * p <0.01).