. Author manuscript; available in PMC: 2016 Jan 31.

Published in final edited form as: J Immunol Methods. 2014 Dec 20;417:86–96. doi: 10.1016/j.jim.2014.12.011

Table 2.

Comparison of the OI-RD/microarray-based assay platform with Biacore 3000 in characterization of specific affinity constants and rate constants of monoclonal rabbit antibodies with 6 immobilized peptide targets.

	Biacore 3000			OI-RD scanning sensor

Original proteins from which 6 peptides are derived	k_on 10⁵s⁻¹M⁻¹	k_off 10⁻⁵s⁻¹	K_d pM	k_on 10⁵s⁻¹M⁻¹	k_off 10⁻⁵s⁻¹	K_d pM
CD34	3.5	1.4	41	0.37	0.43	115
Cytokeratin 15	7.3	1.8	247	0.16	0.89	564
EGFR Phospho	0.69	0.058	3.5	0.42	0.08	19
Glucagon	3.7	2.6	70	0.35	1.2	331
Glut-1	4.6	1.1	24	0.36	0.028	7.7
Vimentin	2.3	7.1	306	0.25	4.3	1700