Table 3.
Suppressor mutations in the priA gene, arising in ΔradD ΔrecG (EAW 522) or ΔradD recG– (EAW 368) strains.
Mutation | PriA substitution |
---|---|
Suppressors of ΔradD recG | |
832 T→G | S278A |
1136 G→C | R379P |
2182 G→T | D728Y |
1558 G→C | A520P** |
1470 A→G | T491A |
CGCTGG | IN W689(RW)* |
Suppressors of ΔradD ΔrecG | |
1286 G→C | G429A |
1861 G→C | A621P |
904 G→A | G302F |
1480 C→T | L494F |
1558 G→C | A520P** |
Insertion of two new codons encoding RW, after codon 689. Note that this is a new repetition of codons 686-687 and 688-689, which encode a tandem repeat of the sequence RW (i.e., RWRW→RWRWRW).
Mutation also found previously in response to recG deficiency (Al Deib et al., 1996).