Table I.

Sxi Binding Sites Upstream of 32 Sxi-Induced Genes Ranked by Decreasing fold change

Gene #	Sxi Binding Site (SBS) sequence	bp from ATG	strand +/−	SBS type	Gene Number	Gene Name/Description	Gene Group
1	TTGATTGTTGATGGGCAAAAG	−242	+	I	CNB04250	CPR2/non-MAT pheromone receptor	1
	GTCTTGTGTGAAGCAGATTGG	−578	−	III
2	GTGATGGGAGATAGGGCAAGG	−530	+	I	CNB01320	hypothetical protein	3
3	CTGATTGCTGATCGACGGTAT	−641	−	I	CNJ03000	hypothetical protein	3
	GTGATGACAGATGGCAGAAGT	−388	+	I
4	CTGATTGCGCATTGACGGATG	−331	+	I	CNB01190	CLP1/dikaryon regulator	1
5	CTGATTACTGAAGCAAACAAG	−171	−	I	CNI00810	hypothetical protein	3
6	GTGATTTCTGAACGAAGTTAT	−128	−	I	CNH01950	hypothetical protein	3
	CTGATTGGTGAACGAAGATGT	−97	−	I
7	GTGATGTGACATGGCCGTAGG	−790	+	I	CNF00230	hypothetical protein	3
8	GTGATGACTGATGGAACGATG	−381	−	I	CNL06750	hypothetical protein	3
9	GTGATTTCTGAACGAAGTTAT	−331	+	I	CNH01960	hypothetical protein	3
	CTGATTGGTGAACGAAGATGT	−362	+	I
10	CTGATTGCAGTACGACATAAG	−872	−	I	CNG01240	LAC1/diphenol oxidase	4
11	GTGTTGAGTGATGGAAGTGGT	−114	−	II	CNJ02870	hypothetical protein	3
12	TTGATTGCCCATTGAAGGAAG	−126	−	I	CNI04000	E3 ubiquitin-protein ligase	2
	GTCATTTTACAACGAACAAGG	−323	+	III
13	GTGATTTCCGCTTGAACGAAT	−78	−	I	CNA07770	SPR1/exo-1,3-betaglucanase	2
	CTGTTGGGAGAAAGGCGCGAT	−252	−	II
	GTGTTGTCCGTAGGGAGCTAT	−766	−	II
14	TTGATTAGAGTTGGAGAGAAG	−518	+	I	CNB04840	COX11/cytochrome oxidase factor	2
15	TTGATGTGAGAAGGAAACAAG	−463	−	I	CNB03490	MED1/development regulator	2
16	GCGATCGTTGATGGGAGAAAT	−466	+	III	CNF04150	RPS2/ribosome subunit S2	2
	GTGATTTGCCAAGCGGGGTAT	−703	+	I
17	CTGATGAGAATATCAAGGAGG	−886	−	III	CNE01390	DED81/asparaginyl-tRNA synthetase	2
18	TGGATGGTAGATGGAGAGAAG	−34	−	I	CNA00510	CIT1/citrate synthase	2
19	TTGTTGGCTGTTTGGTGGAAG	−576	−	II	CNA01120	IMA5/alpha-glucosidase	2
20	CTGATTGGTATTCCAAGGGTG	−610	−	I	CNI00410	MPK1/MAP kinase	2
21	CTGATGGCAGAAGCAGCGTAG	−777	−	I	CNJ02220	ANT1/adenine nucleotide transporter	2
22	GAGATTTCTGTTGCAGAAAAG	−154	−	III	CNJ00490	AGO2/argonaute protein	1
23	TTGATGGCGGTACGTAGCAAT	−203	−	I	CNC06460	VAD1/DEAD box protein	1,4
	GTGATTGGAGATCCAAAGGAA	−778	+	I
24	TAGATTTGTGATTGAGGGATT	−22	−	III	CNA01190	oxidoreductase	2
25	GTGATTGGCCCTCGGCGCAAG	−642	−	I	CNJ00690	DAL4/allantoin permease	2
26	GTGATTGGTGTACGAAAATGA	−344	+	I	CNC01910	DUR1/urea amidolyase	2
27	TTGAGGGCAGTTGGAGGAAAT	−693	−	III	CNK02120	CTR2/copper transporter	2
28	GTGCTGGCTGATGCGACTAAG	−144	−	III	CNN01140	CDC53/E3 ubiquitin ligase subunit	2
	TTGTTGAGGCTTTGAAGGAGT	−886	−	II
29	TTCTTTTGAGAAGCCACAAGG	−168	−	III	CNA05290	SCC2/cohesin loading factor	2
	TTGTTGATACATCGAAGGTGG	−904	+	II
30	CTGTTGTCTGATCCGCATCGG	−675	−	II	CNA02070	hypothetical protein	3
	GGGATGGCTGATGGGAGAATG	−864	+	III
31	TGGATTATTGAACGCAAGTTG	−471	−	III	CNF01940	SSM4/E3 ubiquitin ligase	2
32	TTGTTGTGAGAACGATGGGAT	−743	−	II	CNE03890	CCP1/cytochrome c peroxidase	2

Genes are listed in order of decreasing fold change ranging from 20-fold (#1) to less than 2-fold (#32) induction. Bolded sequences refer to conserved in vitro binding sequences for Sxi2a (TGAT) and Sxi1α (AAG or GAAG). SBS types are defined by the sequences of their Sxi2a half-sites as follows: I = TGAT, II = TGTT, III = all other half-sites. Gene groups: 1 = Sexual Development, 2 = Conserved Domains, 3 = Hypothetical Proteins, 4 = Virulence