Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Pediatr Blood Cancer. 2015 Jan 28;62(4):581–586. doi: 10.1002/pbc.25348

Figure 1. GRP-R silencing downregulates HIF-1α and VEGF expression.

Figure 1

A. Western blotting was used to demonstrate decreased HIF-1α and VEGF protein expression in BE(2)-C/shGRP-R cells under normoxic and chemically induced hypoxia using CoCl2 (100 μM). β-actin demonstrated relatively equal loading. B. BE(2)-C/shCON and shGRP-R cells were seeded onto 6-well plates and cultured for 48 h, then supernatant was collected for VEGF ELISA. ELISA was completed in triplicate (means ± SEM; *=p<0.05 vs. shCON).