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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Pediatr Blood Cancer. 2015 Jan 28;62(4):581–586. doi: 10.1002/pbc.25348

Figure 3. Hypoxia and PDK4 regulate the expression of HIF-1α and VEGF.

Figure 3

A. Cells were transfected with non-targeting control or PDK4 siRNA for 24 h, and then were treated with CoCl2 (100 μM) for 24 h. PDK4 and HIF-1α expression were detected by Western blotting. PDK4 silencing decreased CoCl2 induced HIF-1α expression. β-actin demonstrated relatively equal loading. B. VEGF secretion was measured using cell culture supernatant by ELISA. ELISA was completed in triplicate (means ± SEM; *=p<0.05 vs. siNTC).