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. 2015 Feb 24;10(2):e0116758. doi: 10.1371/journal.pone.0116758

Fig 2. Evaluation of OM isolation quality.

Fig 2

Transmission electron microscopy of (A) purified E. ruminantium and (B) the insoluble precipitate after 0.1% sarkosyl treatment; scale bar = 200 nm. (C) SDS-PAGE and (D) Western blot of E (elementary bodies), S (sarkosyl-soluble fraction), and OM (outer membrane fraction) using monoclonal antibodies against Map1. Band 1: Map1-14, X5HG56, GroEL; Band 2: Map1+1, Map1, Map1-6, VirB10, VirB4, GroEL, PyrE, Q5HAR6, X5HG56, 30S-S8; Band 3: Map1+1, Map1-6, Map2, GroEL, PyrE, Q5HAR6, X5HG56, Q5FGC2, Q5HBI2, Q5FHJ9; Band 4: Map1, Map1-6, VirB4, GroEL, DnaK, BamA, FusA, Pnp, Q5HAR6, X5HG56, Q5FH07, Q5HBS6; Band 5: VirB4, VirB10, VirB11, DnaK, HtpG, GroEL, FusA, 30S-S1, Q5FGV5, Q93FS2; Band 6: Map1, Map1-14, VirB10, PleD, GroEL, DnaK, FtsZ, 30S-S1, Q5HB83, Q5FGA7, Q5HBE1; Band 7: Map1-14, GroEL, DnaK, FtsZ, HtpG; Band 8: Map1-6, Map1, GroEL, DnaK, FtsZ, BamA; Band 9: Map1-6, Map1, Map1+1, Map1-14, GroEL, DnaK, BamA, Q5FFE6, Q5HAR6; Band 10: Map1-11, Map1-13, Map1, Map1+1, Map1-6, VirB10, VirB9, Q5FFE6, Q5HAR6, Q5HBI2, Q5HA95; Band 11: Map1, Map2, BamA, DnaK, GroEL, FusA, Def, 50S-L4, PyrE, X5HG56, Q5HBI2; Band 12: 30S-S18, 30S-S12, 50S-L7/L12, 50S-L18, 50S-L24, 50S-L28 X5HG56, Q5HBN6; Band 13: HupB, X5HG56; Band 14: 30S-S12, 50S-L7/L12, 50S-L18, GroEL, YajC, PyrE.