Fig 2. Growth, attachment, proliferation and apoptosis in standard density calvaria cell cultures of BSP-/- and BSP+/+ mice.

(A) Time-course of in vitro cell growth in BSP+/+ and BSP-/- MCC cultures N = 12 dishes/time point. (B) Number of adherent BSP-/- and BSP+/+ MCC 30 min, 1 hr and 2 hrs after plating. N = 4 dishes/group. (C and D) In vitro proliferation in BSP+/+ and BSP-/- MCC cultures assayed by BrdU incorporation (C, N = 12 dishes/group) and KI67 labelling (D, N = 6 coverslips/genotype/time-point, 10 fields analysed/coverslip, results expressed as number of labelled cells/1000). (E) In vitro apoptosis in BSP+/+ and BSP-/- MCC cultures assayed by TUNEL at D6. **: p< 0.01, ***: p< 0.001 vs BSP+/+; Mann-Whitney U Test and (D) contingency table with χ₂ test.