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. 2015 Feb 24;10(2):e0117402. doi: 10.1371/journal.pone.0117402

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© 2015 Bouet et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Micrographs (Insets: higher magnification) and (B) quantification of total mineralized colonies formed at D14 in BSP+/+ and BSP-/- MCC cultures grown at standard (5000 cells/cm²) and high density (25000 cells/cm²). N = 3 dishes/group. Expression of osteoblast-associated (C and D) and SIBLING genes (E and F) at D14 in BSP+/+ (C, E) and BSP-/- (D, F) cultures. N = 3 dishes/group. Quantification of OPN in the cell layer (G) and the culture medium (H) of BSP+/+ and BSP-/- standard and high density cultures. Results are normalized on total cell layer proteins. N = 6 dishes/group. *: p< 0.05, **: p<0.01 vs BSP+/+; #: p< 0.05, ##: p<0.01 vs standard density. Mann-Whitney U Test.