Fig. 3.

Immunolocalization of LM2, LM14, and MAC207 epitopes in the rhizodermis of barley cv. Karat and the rhl1.b mutant. (A, C, E, P, R, T) Autofluorescence illustrates cell patterning in the mature root hair zone. Fluorescence labelling of AGP epitopes in (B, D, F) cv. Karat and (Q, S, U) the rhl1.b mutant, with (B, D, F, Q, S, U, G–O, V–X) showing subcellular localization based on immunogold labelling. (A–F) Epitopes were more abundant in the trichoblasts and root hair tubes than in the atrichoblasts. (G–O) In the trichoblast cell wall, LM2, LM14, and MAC207 epitopes were only detected in the wild-type cultivars (H–X). In the root hairless mutant, the three epitopes were homogeneously distributed within the epidermis. Asterisks, root hair tubes; arrowheads, trichoblasts; arrows, gold particles; CW, cell wall; Cyt, cytoplasm. Scale bars: (A–F and P–U) 50 µm; (G–O and V–X), 100nm.