Table 3.
AGP epitopes detected in the roots of various plant species
| mAbs | Recognized epitope | Epitope expression in roots |
|---|---|---|
| JIM4 | β-D-GlcA-(1,3)-α-D-GalA-(1,2)-α-L-Rha (Yates et al., 1996) | Carrot: protoxylem, pericycle (Knox et al., 1989
1991; Casero et al., 1998) Radish: individual pericycle cells (Casero et al., 1998) Onion, pea: lack of expression in all root tissues (Casero et al., 1998) Barley: lack of expression in all root tissues (this work) |
| JIM8 | Unknown | Maize: protophloem sieve elements (Samaj et al., 1998) Wax gourd: protoxylem (Xie et al., 2011) Barley: endodermis and metaphloem sieve elements (this work) |
| JIM13 | β-D-GlcA-(1,3)-α-D-GalA-(1,2)-α-L-Rha (Yates and Knox, 1994; Yates et al., 1996) |
Arabidopsis: xylem (Dolan et al., 1995); root cap and all root apex cells (Vicre et al., 2005) Carrot: early stage of xylem development (Knox et al., 1991) Maize: pericycle, protophloem sieve elements, companion cells, root cap (Samaj et al., 1998) Wax gourd: lack of expression in all root tissues (Xie et al., 2011) Barley: all root cells, stronger in epidermis, endodermis, and metaphloem sieve elements (this work) |
| JIM14 | Unknown |
Arabidopsis: all root cells, stronger in metaphloem sieve elements (Dolan and Roberts, 1995) Carrot: all root cells (Knox et al., 1991) Wax gourd: lack of expression in all root tissues (Xie et al., 2011) Barley: metaphloem sieve elements (this work) |
| JIM15 | Unknown | Carrot: all root cells, except epidermis (Knox et al., 1991) Wax gourd: lack of expression in all root tissues (Xie et al., 2011) Barley: lack of expression in all root tissues (this work) |
| JIM16 | Unknown | Carrot: all cells in root meristem (Knox et al., 1989) Wax gourd: all root cells, weaker in cortex and parenchyma (Xie et al., 2011) Barley: endodermis (this work) |
| JIM17 | Unknown | Wax gourd: lack of expression in all root tissues (Xie et al., 2011) Barley: lack of expression in all root tissues (this work) |
| LM2 | β-Linked GlcA (Smallwood et al., 1994) |
Arabidopsis: epidermis, weaker in trichoblasts (Andeme-Onzighi et al., 2002) Maize: surface of root hair tubes (Samaj et al., 1999) Wax gourd: all root cells, except root epidermis (Xie et al., 2011) Barley: all cells except xylem, diverse expression in epidermal and parenchyma cells (this work) |
| LM14 | Arabinose- and galactose-enriched carbohydrate chains (Moller et al., 2008) | Wax gourd: all root cells, stronger in epidermis (Xie et al., 2011) Barley: companion cells of phloem and diverse expression in epidermis (this work) |
| MAC207 | β-GlcA-(1,3)-α-GalA-(1,2)-Rha (Bradley et al., 1988; van den Bosch et al.,1989) |
Arabidopsis: root cap and all root apex cells (Vicre et al., 2005) Carrot: all cells in root meristem (Knox et al., 1989) Wax gourd: lack of expression in all root tissues (Xie et al., 2011) Barley: companion cells of phloem and diverse expression in epidermis (this work) |