Fig. 7.

The dsptp1 mutant shows altered expression of several dehydration-responsive genes after osmotic stress: DREB2A (A), RD22 (B), ICK (C), ABF (D), COR15A (E), and ERD1 (F). The expression level of these genes in response to stress was analysed by qRT-PCR. Total RNA was isolated from 15-d-old WT, Col0, and dsptp1 seedlings treated with 385mM mannitol for 0h, 2h, 8h, and 12h. In all experiments, the expression of the constitutive Actin2 gene was used as the control. Three replicates were made for each treatment with similar results. Values are mean ± SE of three different experiments. Means denoted by the same letter do not significantly differ at P < 0.05 according to Duncan’s multiple range test.