Fig. 8.

DsPTP1 is involved in osmotic stress- and ABA- signalling. (A) Percentage Arabidopsis seed germination with WT, dsptp1, OE, and Com lines in MS medium supplemented with or without 0.5, 1, and 2 μM ABA. (B) Seedlings were grown in MS medium for 1 week and then transferred to MS medium with or without 5, 10, 20, and 50 μM ABA. Root elongation (increase in length after transfer) was determined after 7 d. Error bars indicate SE (n = 15 for root elongation; n = 3 for germination; each experiment contained 100 seeds). Three replicates were made for each treatment with similar results, and graphs correspond to one representative experiment.

(C) Determination of endogenous ABA level in WT and dsptp1 seedlings after mannitol treatments. Expression differences of the ABA-biosynthesis key genes ZEP, NCED, AAO3, and catabolic gene CYP707A3 and CYP707A4, in WT and dsptp1 were analysed before or after osmotic stress. Values are mean ± SE of three different experiments. Means denoted by the same letter do not significantly differ at P < 0.05 according to Duncan’s multiple range test.