Fig. 4.

Manipulation of apoplastic fluid carbon availability alters MpNEP2 expression and the necrosis rate of infected tissues. (A) Time (in days ater infection) that necrotic symptoms took to cover 50% of the length of infected stems after infiltrations with solutions containing carbon (sucrose, glucose, and fructose at 10mM each), carbon with 400ng ml^–1 rapamycin, and rapamycin only. (B) MpNEP2 gene expression assayed at 10 d intervals after infiltration of infected stems. Data points represent the mean of four biological replicates with calculated standard deviations (unpaired Student’s t-test; *P<0.05; **P<0.005; ***P<0.0005). (This figure is available in colour at JXB online.)