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. 2014 Dec 24;66(5):1437–1452. doi: 10.1093/jxb/eru499

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 8. — DR5:GUS expression in the bases of young (A, C, E, G) or old (B, D, F, H) cuttings treated with 0 µM (A, B), 0.3 µM IAA (C, D), 1 µM IAA (E, F), or 3 µM IAA (G, H) and all stained at 24h after cutting. C, cortex; Ph, phloem; Ca, cambium; X, xylem. Samples were cleared for 45min in acetone on ice, stained in x-gluc for 24h, free hand sections made and photographed mounted in water. Subset panels from same images at higher cellular resolution. Sections were cut from four separate stems for each age/treatment combination. Inset images are of the vascular bundles (containing xylem, cambium, and phloem). Bars in full size panels are 200 µm, bars in the inset panels are 60 µm.