Root-hair density (number per 500 µm) and the effect of GR24 (3×10–6 M) and IAA (5×10–6 M) treatments on root-hair density in Arabidopsis thaliana. (A) WT (Col-0), max2-1, max4-1, eir1-1, eir 1–4, tir3-103, tir3-104, and tir3-105. (B) WT (C24), der1-1, der1-2, and der1-3. Seedlings were grown under high (2mM) and low (1 µM) Pi conditions or supplemented under low-Pi conditions with GR24 or IAA (48 HPG) (n=300). Means±SE are shown. Asterisks indicate significantly different results between hormone treatments (GR24 or IAA) and non-treated seedlings, all grown under low-Pi conditions, in each of the examined lines. Ŧ indicates that means are significantly different between WT under low-Pi conditions and mutant lines treated with hormones (GR24 or IAA) or non-treated plants, all under low-Pi conditions, as determined by a Student’s t-test (P≤0.05). Different lower-case letters above the bars indicate statistically significant differences between means of WT and mutant-line seedlings grown under high-Pi conditions by a one-way ANOVA pairwise multiple-comparison Tukey–Kramer test (P≤0.05). RH, root hair.