Fig. 2.

ABA attenuates the enhancing effect of various dormancy release stimuli. (A) In the combined ABA–HC treatment, buds were treated with ABA for 48h prior to HC treatment. All other details are as in Fig. 1. The experimental scheme used in (B), (C), and (D) is identical to that described in (A) apart from the indicated changes. (B) HC treatment was replaced with HS treatment where cuttings were immersed in 50 °C water for 1h as detailed in the Materials and methods. (C) HC treatment was replaced with AZ treatment where cuttings were sprayed with 2% sodium azide and 0.02% Triton. (D) HC treatment was replaced with hypoxia treatment where cuttings were incubated for 48h in sealed jars which were flushed with N₂ to reduce O₂ level to 1%.