Fig 1. Laser capture microdissection (LCM) of fibrotic and non-fibrotic bronchi.

Microdissection of fibrotic bronchial lesions, such as that indicated by the circled area on the right, was performed on frozen sections. Similarly, exposed but non-fibrotic bronchi, such as the two noted on the left, were microdissected and collected separately. RNA was subsequently extracted from both the fibrotic and the non-fibrotic bronchial specimens, and utilized for microarray and PCR analysis. 4x original objective magnification.