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. 2015 Mar;161(Pt 3):516–527. doi: 10.1099/mic.0.000027

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© 2015 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 7. — Composition analysis of B. burgdorferi indicates the distinction between the different pleomorphic forms and presents atypical cell wall characteristics. Live spirochaetes, blebs observed in normal culture conditions, 2 h H₂O RBs and BFL aggregates in suspension as well as 24 h doxycycline-treated damaged cells were stained with PI, WGA conjugated with Alexa 555 (WGA 555) and BODIPY to indicate DNA, polysaccharides and lipids. Acid fuchsin was used for methanol-fixed cells to stain collagen. Upper panel visualizes the cells imaged with confocal laser scanning microscopy. Lower panel represents the morphology of the cells with DIC. Bars, 5 µm.