Fig. 5.

The effect of LY294002 on the expression of SR-A, CD36 and LPL mRNA in HMDM. HMDM were incubated for 24 h with the DMSO vehicle or 10 μM LY294002 (empty bars) or 100 μM LY294002 (filled bars). Total RNA was subjected to real-time quantitative PCR using primers against SR-A, CD36 or LPL as indicated. The mRNA expression levels were calculated using the comparative C _t method and normalized to RPL13A with vehicle-treated cells given an arbitrary value of 1. Data represent means ± SD from three independent experiments. Statistical analysis was performed using the Student’s t test, ***P < 0.001