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. 2015 Feb 25;5:8571. doi: 10.1038/srep08571

Figure 2. Whole-mount smFISH for endogenous genes in organs/tissues.

Figure 2

(a) Filtered and threshold applied single plane confocal images of the indicated tissues of embryos subjected to the whole-mount smFISH for endogenous genes specifically expressed in the organs/tissues. The boxed areas are shown as higher magnification at the right top corner in each panel (top rows). The organs/tissues where the genes are known not to be expressed are shown as negative control (bottom rows). (b) Filtered and threshold applied single plane confocal images of the indicated tissues of embryos subjected to the whole-mount smFISH protocol for ubiquitously expressed genes. The boxed areas are shown as higher magnifications at the right top corner in each panel. In both (a) and (b), shown are confocal images taken with intact whole-mount embryos, except the panels labeled as “brain” that are taken with the head tissue separated from the rest of the body.