Table 1. Summary of mant-guanine nucleotide kinetics and affinities to EF4 and variants (20°C).
| koff (GDP) (s−1) | koff (GTP) (s−1) | kon1 (GDP) (μM−1s−1) | kon1 (GTP) (μM−1s−1) | kon2 (GDP) (μM−1s−1) | kon2 (GTP) (μM−1s−1) | KD Mant-GDP (μM) | KD Mant-GTP (μM) | |
|---|---|---|---|---|---|---|---|---|
| Wild Type | 4.2 ± 0.3 | 21 ± 4 | 1.0 ± 0.1 | 0.4 ± 0.1 | 6.4 ± 0.6 | 5.5 ± 1.4 | 5 ± 1 | 23 ± 11 |
| ΔA494 | 2.9 ± 0.5 | 28 ± 5 | 0.8 ± 0.1 | 0.3 ± 0.1 | 5.1 ± 0.2 | 5.4 ± 0.5 | 3 ± 1 | 18 ± 5 |
| ΔP520 | 3.4 ± 0.1 | 34 ± 12 | 0.9 ± 0.1 | 0.3 ± 0.1 | 7.4 ± 0.4 | 9.6 ± 1.0 | 5 ± 1 | 24 ± 5 |
| ΔG555 | 4.9 ± 0.9 | 14 ± 4 | 1.0 ± 0.1 | 0.4 ± 0.1 | 3.3 ± 0.4 | 7.9 ± 0.8 | 4 ± 1 | 32 ± 11 |
| H81A | 2.8 ± 0.3 | 29 ± 1 | 0.7 ± 0.1 | 0.2 ± 0.1 | 2.3 ± 0.3 | 4.7 ± 1.1 | 6 ± 1 | 16 ± 9 |
| EF-Ga | 300 | 7 | n.d. | 0.6 ± 0.1 | n.d. | n.d. | 17 | 7 |
a) 13.