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. 2015 Feb 25;5:8573. doi: 10.1038/srep08573

Table 2. Intrinsic and 70S ribosome-stimulated GTPase activity of EF4 and variants.

  KM (μM) kcat (s−1) kcat/KM (s−1M−1)
  Intrinsic 70 S Intrinsic 70 S Intrinsic 70 S
EF4 270 ± 90 0.32 ± 0.08 0.003 ± 0.001 2.3 ± 0.3 11 ± 5 7 ± 2
ΔA494 370 ± 35 36 ± 26 0.005 ± 0.001 5 ± 3 14 ± 3 0.14 ± 0.13
ΔP520 900 ± 200 10 ± 5 0.007 ± 0.001 5 ± 2 8 ± 2 0.5 ± 0.3
ΔG555 700 ± 100 7 ± 3 0.003 ± 0.001 0.6 ± 0.2 4 ± 2 0.09 ± 0.05
EF-G n.d. 0.40 ± 0.10 n.d. 2.7 ± 0.3 n.d. 7 ± 2