Table 1. COLEC10 and COLEC11 primer sets.
| Forward primer | Reverse primer | Internal primer | |
|---|---|---|---|
| COLEC10 | |||
| Promoter region | |||
| Fragment 1 | 5′-cctttcacctaactcttagttga-3′ | 5′-gcctttctggtacactatctc-3′ | |
| Fragment 2 | 5′-ttcgcctttctgccacatcc-3′ | 5′-gaactccagtgtgcatgtcag-3′ | |
| Fragment 3 | 5′-tcaagtactcagcagaaggca-3′ | 5′-caaagccattcattgctgtgg-3′ | |
| Exonic regions | |||
| Exon 1 | 5′-ggtccatttggagcactgag-3′ | 5′-ataagtaacagaccagatctgg-3′ | |
| Exon 2 | 5′-aagtgcatgtgaacatacatcc-3′ | 5′-atgcaacttgagaaacatatgag-3′ | |
| Exon 3 | 5′-tagtatatattcatactcactcac-3′ | 5′-ccaccatgcccagctaattc-3′ | |
| Exon 4 | 5′-ttatgactcatttaattagttgcac-3′ | 5′-actgccagagttttctcttgg-3′ | |
| Exon 5 | 5′-tttagaattgtcttatagttcattc-3′ | 5′-cagacttgaggtgtcctagg-3′ | |
| Exon 6 | 5′-tctgtgactacctcataatagg-3′ | 5′-tgtaacctggcgagttattgg-3′ | |
| 5′-gagtgccatcttaccatgtac-3′ | 5′-aaggagaacttccaagtatagc-3′ | ||
| Pyrosequencing | |||
| –7C>T | 5′-tgtgtgttccaaatacttccc-3′ | 5′-[B]gactctgaatttgcaaaagaaatag-3′ | 5′-tgggagacccttttctgagg-3′ |
| Glu78Asp | 5′-acattagccacatttcaaggga-3′ | 5′-[B]acctcggtctctgagtgttg-3′ | 5′-gtttgcttttcaggaattaaagg-3′ |
| Arg125Trp | 5′-[B]cgtgtcttcatcggcatca-3′ | 5′-ctccttgtcaaactcacacatga-3′ | 5′-ccacttgttgaaggtccgca-3′ |
| COLEC11 | |||
| Promoter region | |||
| Fragment 1 | 5′-tgtctggttttggtatcagggt-3′ | 5′-gcttctatgcagcccgaatc-3′ | |
| Fragment 2 | 5′-tcacctttgaacctagcttctta-3′ | 5′-cctggggagtgtgggaac-3′ | |
| Fragment 3 | 5′-ttgggatgttcgggttggag-3′ | 5′-atcaaactttggaactctggtg-3′ | |
| Exonic regions | |||
| Exon 1 | 5′-ttgggatgttcgggttggag-3′ | 5′-atcaaactttggaactctggtg-3′ | |
| Exon 2 | 5′-ttggtgcctggtgctgacc-3′ | 5′-ggaattccatggccgaagac-3′ | |
| Exon 3 | 5′-aaacacaggccctttctaagag-3′ | 5′-ccacaggtcagcacaaggag-3′ | |
| Exon 4 | 5′-ctccattctatagtgtgtatgac-3′ | 5′-agctgcagcccctccatcc-3′ | |
| Exon 5 | 5′-aaagaatgatcactcgataatcc-3′ | 5′-gtgtccaccgtgggatgtg-3′ | |
| Exon 6 | 5′-ttgaaatacatgtgtctgccct-3′ | 5′-gcacagccacaggcagcag-3′ | |
| Exon 7 | 5′-acctcccagccctgtcctg-3′ | 5′-cattttctcagtttagacaacagc-3′ | |
| 5′-cccaacaatgcctacgacg-3′ | 5′-catggataatagtgtgaaggaacc-3′ | ||
| Pyrosequencing | |||
| His219Arg | 5′-[B]tggaaagagagcaaatgttcct-3′ | 5′-actggaattgtagtgttgatgga-3′ | 5′-actaatatccagttgtccaacaaa-3′ |
Forward and reverse primers were used for PCR amplification, internal primer was used for pyrosequencing. All forward primers for the promoter and exonic regions include a 5′-T7 sequence (5′-TAATACGACTCACTATAGGG-3′). Biotinylated oligos are indicated as [B].